For 1 L of media:

• 47.6 TB mix
• 4 ml glycerol

Add water to 1 L and autoclave at 121°C for 15–20 minutes.

After autoclave add:

• 0.1% - 1% glucose
• 10 mM MgSO₄
• antibiotics

For 1 L of media:

• 10 gr Yeast extract
• 20 gr Peptone
• 80 mg Adenine sulfate
• 20 gr Dextrose (Glucose)

For plates add:

• 20 gr Agar

For 1 L of media:

• 1.92 gr drop-out mix
• 6.7 gr yeast nitrogen base w/o AA
• 20 gr carbohydrate (glucose/galactose)

For plates add:

• 20 gr Agar

Keep refrigirated
Shake well before using

• 50 mM Tris-HCl pH 8.0
• 10 mM EDTA
• 100 µg/ml RNase A
• 43 µg/ml Thymophthalein (blue pH indicator)

1000X Blue pH indicator dye:
43 mg/ml Thymophthalein in 100% ethanol

• 200 mM NaOH
• 1% SDS

Neutralization buffer for spin columns

• 4.2M guanidine hydrochloride (GuHCl)
• 0.9M potassium acetate pH 4.8

Neutralization buffer for bacmid purification, midi, maxi, giga preps
Do not use with spin columns!

• 3.0 M potassium acetate pH 5.5

extra wash step for EndA+ removal or PCR purification

• 5M guanidine hydrochloride (Gu-HCL)
• 30% isopropanol

• 5.5 M guanidine thiocyanate (GuSCN)
• 20 mM Tris-HCl, pH 6.6 (25ºC)

• 80% ethanol
• 10mM Tris-HCl pH 7.5 (25ºC)

• 200 mM HEPES pH 7.5
• 1.5 M NaCl