Media recipes Terific Broth (TB) YPAD Drop-out media Back to Protocols Terific Broth (TB) For 1 L of media: 47.6 TB mix 4 ml glycerol Add water to 1 L and autoclave at 121°C for 15–20 minutes. After autoclave add: 0.1% - 1% glucose 10 mM MgSO4 antibiotics YPAD For 1 L of media: 10 gr Yeast extract 20 gr Peptone 80 mg Adenine sulfate 20 gr Dextrose (Glucose) For plates add: 20 gr Agar yeast drop-out media For 1 L of media: 1.92 gr drop-out mix 6.7 gr yeast nitrogen base w/o AA 20 gr carbohydrate (glucose/galactose) For plates add: 20 gr Agar
