Lab Protocols

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Protocols

Internal lab protocols — Alon Lab members only.

Orders

Orders / Inventory Excel Sheet

SharePoint

Orders

Logins

Internal Page

Primers

Alon Lab Primer List

SharePoint

Primers

How to Order Primers

Internal Page

Calculator

Calculate OD and When to Harvest

Streamlit App

Calculator

Calculate NEB HiFi Assembly

Streamlit App

Calculator

Calculate Nanobody Library Diversity

Streamlit App

Mol Bio

Miniprep

PDF Protocol

Mol Bio

DNA Gel Extraction

PDF Protocol

Mol Bio

Reaction Cleanup

PDF Protocol

Mol Bio

KAPA PCR

PDF Protocol

Mol Bio

KLD Kit

PDF Protocol

Mol Bio

DNA Extraction from Baculovirus

PDF Protocol

Mol Bio

Media and Buffer Recipes

Internal Page

Purification

Purification from Periplasm

PDF Protocol

Purification

Nitrogen Cavitation

PDF Protocol

Purification

Using the Microfluidizer

PDF Protocol

Purification

Coupling Proteins to Sepharose CNBr

PDF Protocol

FPLC

Using the Sample Pump

PDF Protocol

Radioactive

Using the Brandel Harvester

PDF Protocol

Radioactive

Radioactive Swipe Test Form

PDF Protocol

Radioactive

Preparing G-50 Desalting Columns

PDF Protocol

Radioactive

Extract CPM from LS6500

Streamlit App

Radioactive

CPM to nM Calculator

Streamlit App

Cell Culture

Fectopro Transfection

PDF Protocol

MultiBacMam

DH10EMBac Transformation

PDF Protocol

MultiBacMam

Isolation of Bacmid DNA

PDF Protocol

MultiBacMam

Bacmid Transfection

PDF Protocol

Crystallography

Preparing 9.9 MAG for LCP

PDF Protocol

Crystallography

Pointers for Other MAG

PDF Protocol

Crystallography

Gryphon Calibration

PDF Protocol

Crystallography

Gryphon LCP Setup

PDF Protocol