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Protocols

Orders
  • Orders/inventory excel sheet
  • Logins
Primers
  • Alon Lab Primer List
  • How to order primers
Calculators
  • Calculate OD and when to harvest
  • Calculate NEB HiFi Assembly
  • Calculate nanobody library diversity
Molecular biology
  • Miniprep
  • DNA gel extraction
  • KAPA PCR
  • KLD kit
  • Media and buffer recipes
Protein purification
  • Purification from periplasm
  • Nitrogen cavitation
  • Using the microfluidizer
  • Coupling proteins to sepharose CNBr
FPLC
  • Using the sample pump
Radioactive binding
  • Using the Brandel harvester
  • Radioactive swipe test form
  • Extract CPM from LS6500
Cell Culture
  • Fectopro Transfection
MultiBacMam
  • DH10EMBac transformation
  • Isolation of bacmid DNA
Crystallography
  • Preparing 9.9 MAG for LCP
  • Pointers for other MAG
  • Gryphon calibration
  • Gryphon LCP setup
Alon lab, SHM-B323, 333 Cedar St., New Haven, CT 06510
Department of Pharmacology Yale School of Medicine
© Assaf Alon 2024