MultiBacMam

• DH10EMBac transformation
• Isolation of bacmid DNA

Calculators

• Calculate nanobody library diversity
• Calculate NEB HiFi Assembly

Molecular biology

• Miniprep
• KAPA PCR
• KLD kit
• Media recipes

Protein purification

• Purification from periplasm

FPLC

• Using the sample pump

Radioactive binding

• Using the Brandel harvester
• Radioactive swipe test form

Cell Culture

• Fectopro Transfection

Crystallography

• Preparing 9.9 MAG for LCP
• Pointers for other MAG
• Gryphon calibration
• Gryphon LCP setup