Protocols MultiBacMam DH10EMBac transformation Isolation of bacmid DNA Calculators Calculate nanobody library diversity Calculate NEB HiFi Assembly Molecular biology Miniprep KAPA PCR Media recipes FPLC Using the sample pump Radioactive binding Using the Brandel harvester Cell Culture Fectopro Transfection Crystallography Preparing 9.9 MAG for LCP Pointers for other MAG Gryphon calibration